›› 2011, Vol. 23 ›› Issue (6): 456-459.doi: 10.3969/j.issn.1004-616x.2011.06.013

• 核辐射安全专家论坛 • 上一篇    下一篇

X射线对人肺癌A549 细胞Bmi-1表达的影响

梁小芳,邹 跃,江其生,李峰生,王 璐,周湘艳   

  1. 1. 中国人民解放军第二炮兵总医院，北京 100088； 2. 苏州大学临床医学院，江苏 苏州 215123；3. 复旦大学附属肿瘤医院，上海 200032； 4. 中国疾病预防控制中心辐射防护与核安全医学所，北京 100088
  • 收稿日期:2011-09-19 修回日期:2011-09-21 出版日期:2011-11-30 发布日期:2011-11-30
  • 通讯作者: 邹 跃

Effects of X-rays on the expression of Bmi-1 gene in human lung adenocarcinoma cell line A549

LIANG Xiao-fang，ZOU Yue，JIANG Qi-sheng，LI Feng-sheng，WANG Lu，ZHOU Xiang-yan   

  1. 1. Second Artillery General Hospital of PLA, Beijing 100088; 2. Suzhou University Medical College, Suzhou 215123，Jiangsu; 3. Fudan University Shanghai Cancer Center, Shanghai 200032; 4. National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China
  • Received:2011-09-19 Revised:2011-09-21 Online:2011-11-30 Published:2011-11-30
  • Contact: ZOU Yue

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摘要： 目的： 研究X射线照射对人肺癌A549细胞株中Bmi-1 mRNA和蛋白表达的影响。方法：用不同剂量(0、2、4、6 Gy)的X射线照射体外培养的人肺癌A549细胞株，分别采用实时荧光定量PCR和Western blot技术检测照射0、6、12、24、48 和72 h 后Bmi-1 mRNA和蛋白的表达水平。结果：与对照组比较，2、4、6 Gy X射线照射A549细胞后48 h内Bmi-1 mRNA表达升高，差异均有统计学意义(P<0.05)；(2 Gy 48 h组除外)，2 Gy组照射后6 h、4 Gy组12 h、6 Gy组 24 h升高最显著(P<0.05)。照射后48 h内蛋白表达升高(4 Gy除外)，48 h后蛋白表达逐渐下降，至72 h时接近未照射组水平。各时间点(除4 Gy 48 h和72 h外)的蛋白表达与对照组相比差异均有统计学意义 (P<0.05)。结论：在本实验条件下，2~6 Gy剂量X射线照射48 h内可使人肺癌A549细胞Bmi-1表达升高，之后表达逐渐降低。

关键词: 肺癌, Bmi-1, A549细胞, X射线

Abstract: OBJECTIVE: To study the dose and time effects of X-rays on the expressions of Bmi-1 gene and protein in human lung adenocarcinoma cell line A549. METHODS：A549 cell line was exposed to different doses of X-rays (2，4，6 Gy )and the relative level of Bmi-1 mRNA and protein was detected by using quantitative real-time PCR and Western blot at 6，12，24，48，72 h after irradiation. The untreated A549 cells were used as control. RESULTS： Bmi-1 mRNA in A549 cells was highly regulated after irradiated with 2，4，6 Gy and the peaked at 6 h，12 h and 24 h respectively. The expression of Bmi-1 mRNA，except for 2 Gy group，after 48 h showed statistical difference compared with the untreated group(P<0.05). After radiation， the expression of Bmi-1 protein was significant at most time points (P<0.05)，increased during 48 h，but was decreased after 48 h，and approached level of the untreated at 72 h. CONCLUSION：Under our experimental conditions，2-6 doses of X-rays radiation could increase the expressions of Bmi-1 mRNA and protein in A549 cells .

Key words: lung cancer, Bmi-1, A549 cells, X-rays

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